Vol. 9, Issue 11, Part B (2025)

The present study was designed to identify the phytochemical profile and yield of Tinospora cordifolia stem aqueous extracts obtained by Soxhlet extraction and Boiling methods. The Soxhlet extraction had a higher percentage yield (14.89%), when compared to the Boiling method (6.7%). Further, GC-MS analysis of the Soxhlet extract identified 19 phytoconstituents viz., alcohols, esters, alkaloids, fatty acids and phenolic compounds. The major compounds were Glycerin (25.08%), Inositol, 1-deoxy-(15.81%) and 4H-Pyran-4-one, 2, 3-dihydro-3, 5-dihydroxy-6-methyl-(9.79%), along with other potent bioactive compounds such as Desulphosinigrin and Columbin. These metabolites have been reported to exhibit diverse pharmacological activities such as antioxidant, anti-inflammatory, anti-diabetic, anti-hypertensive and anti-cancer effects. On contrary, the extract obtained by Boiling method contained 13 compounds, with Inositol, 1-deoxy-(75.30%) being the major compound followed by other polar carbohydrate derivatives. In conclusion, the Soxhlet extraction method not only enhanced the yield but could also extract a diverse spectrum of therapeutically significant phytochemicals. The presence of compounds such as columbin, desulphosinigrin and ferulic acid derivatives deciphers the potential of T. cordifolia stem as a promising source in the treatment and management of oxidative stress, metabolic disorders, inflammation and cancer.