Vol. 9, Issue 10, Part I (2025)

An attempt was made to investigate the profile of blood biochemicals and enzymes in Jaffarabadi buffalo calves. The study involved 24 calves and was conducted at the Cattle Breeding Farm, Kamdhenu University, Junagadh, following approval from the Institutional Animal Ethics Committee. Iso-managerial condition was ensured. Pelleted concentrate was offered to meet protein requirements feeding standards. A total 3 blood collections from each calf, at 8^th, 91^st and 182^nd day of age were made. The blood biochemicals viz., glucose, cholesterol, triglycerides, total protein, albumin and globulin, blood urea nitrogen (BUN), bilirubin, creatinine, insulin like growth factor (IGF-1): and the enzymes like lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and asparate amino-transferase (AST) and antioxidants like super-oxide dismutase (SOD) and catalase activity were measured using standard kit method. The alterations in the biochemicals and enzymes up to 6 months of age, early (day-8 to 91) and late (day-91 to 182) post-natal phases, were ascertained. Overall mean values of glucose, cholesterol, triglycerides, total protein and albumin were 74.56±1.83 mg/dL, 77.00±1.24 mg/dL, 29.37±0.52 mg/dL and 7.15±0.03 g/dL and 3.34±0.07, respectively. The levels of glucose, cholesterol, triglycerides, and albumin marginally, non-significantly declined by -8.75, -7.81, -14.90, -10.38 respectively; however, these were within the normal physiological range. Total protein and globulin levels increased by 1.90% and 11.84%, respectively. Serum urea nitrogen, bilirubin and creatinine averaged 13.84±0.20,0.17±0.01 and 1.17±0.03 mg/dL, the levels remained almost static and in normal range. The overall mean of the IGF-1 was 45.05±2.65 ng/ml. A highly significant (p<0.001) elevation of 45.11 and 33.39% was observed in serum IGF-1 level during 8 to 91 and 91 to 182 days, respectively, total rise being 93.56%. The enzyme levels lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and asparate amino-Transferase (AST) and antioxidant like super-oxide dismutase (SOD) non-significantly marginally varied but within the normal physiological range. Corresponding overall values were 411.97±2.04,137.20±1.38, 68.60±0.65 and 20.63±0.08 IU/L, respectively. The catalase activity concentration 51.04±0.27, 54.92±1.24 and 56.79±1.77ng/ml at the 8^th, 91^st and 182^nd day of age, overall mean being 55.02±1.38 ng/ml. A highly significant differences (p≤0.001) were observed among all the age groups. An elevation of 7.59 and 3.41% was observed in serum catalyse level during 8 to 91 and 91 to 182 days, total rise being 11.26%, but was within the normal physiological range.