Vol. 9, Issue 10, Part H (2025)

A total of 60 suspected crossbred cows were screened for babesia bigemina. Out of which 40 were positive by molecular study in and around Namakkal district. Animals with the history of fever, weakness, hemoglobinuria anaemia, and jaundice, pale mucus membrane were observed. Haematological and biochemical analysis were done for assessment of infection status and therapeutic efficacy. Giemsa-stained blood smear revealed numerous B bigemina is paired parasites lying almost parallel or at an acute angle to each other. The blood picture and serum biochemistry showed anemia with leucopenia, leukocytosis, neutrophilia and eosinophilia, hypoproteinemia, hypoalbuminemia and elevated liver enzyme (AST). For further confirmation PCR was performed in whole blood using the primer specific for Bb18S rRNA gene (504 bp). The animals were treated with inj. diminazene aceturate (Berenil) is administered at 3.5 mg/kg, i/m (once) and Inj. Oxytetracycline @ 22 mg/kg i/v with Dextrose normal saline and along with supportive for 4 days. Topical synthetic pyrethroids (Flumethrin 1%) also recommended as tick control measures. The animal recovered uneventfully after 7 days of treatment.