Vol. 9, Issue 10, Part H (2025)

Subclinical ketosis (SCK) is a prevalent metabolic disorder in high-yielding dairy cattle during the early postpartum period. This study aimed to evaluate the hemato-biochemical and mineral profile changes in crossbred cows affected with SCK during the periparturient period. A total of 101 cows were screened, among which 8 were diagnosed with SCK based on blood β-hydroxybutyrate (BHBA) >1.2 mmol/L and non-esterified fatty acids (NEFA) >0.4 mmol/L. Blood samples were collected during three physiological stages: far-off dry (FOD), close-up dry (CUD), and fresh. Hematological, biochemical, and mineral parameters were analyzed using automated analyzers. Hematological indices such as hemoglobin, PCV, and TEC showed non-significant declines, while leukocytosis was observed at the fresh stage. Significant decreases in total plasma protein and glucose levels were noted in the fresh stage, while NEFA and BHBA levels increased. Plasma mineral concentrations revealed non-significant variations, although trends suggested decreased calcium and phosphorus levels in fresh stage cows. These findings support that SCK is associated with marked metabolic disturbances that can be used for early detection and management.