Vol. 9, Issue 10, Part G (2025)

Background: Travellers Diarrhoea is a common disease among young adults travelling from cold or temperate countries to hot tropical countries characterised by loose stools 2-3 times per day often resolve by its own within 2 days. Common aetiology is found to be enterotoxigenic E coli bacteria, stress etc.

Objective: To evaluate the effect of temperature on activity of ALT, LDH to extrapolate effects of temperature on human enzyme activity.

Methods: Serum from left over samples of patients with normal and abnormal levels of ALT and LDH were separated stored at 8, 37 and 50 degree Celsius respectively for 4 hours. The test is performed after 4 hours in automated biochemical analyzer Mindray BS600 and the results were recorded. Results were plotted against the temperature in graph and studied.

Results: From the graph we found that in the sample store at 8 degree Celsius, the activity of LDH and ALT increased consistently with concentration of enzymes in the blood. But in samples stored at 37 and 50 degree Celsius there were some variations. Also activity of LDH incresesd till 37 degree Celsius and decreased till 50 degree Celsius whereas activity of ALT gradually decreased with increasing temperature.

Conclusion: Traveller’s diarrhoea is caused due to sudden change in temperature which results in abrupt increase in enzyme activity in intestinal cells stimulating release of ions and water into the gut. Travellers disease is a personalised disease that depends on an individuals enzyme adaptability, concentration in the blood and stability. Young adults are often affected as thermal adaptability of enzymes may not be achieved in young and first time travellers. Traveller’s headache is caused due to decreased emptying of sinusoidal secretion due to sudden decrease in temperature. Change in temperature and climate also trigger enterogenic microbes to increase or decrease in activity causing infections. So the travellers are advised to get acclimatised to the climate or conditions of the destination country before travelling to those countries. But the current study lacks quantitative data of the intestinal enzymes to explain the phenomena. Also the study need to be extended with quantitative enzymatic study in both climatic conditions and also bacteriological study of gut microbiota in Travellers from both the climatic conditions which requires more funding.

Conflicts of Interest: Author certify that there is no involvement of any third party funding or interest in this Research.