Vol. 9, Issue 10, Part G (2025)

The study involved the effect of Andrographis paniculata extract (APE) on p53 expression in liver of cisplatin treated rats. Experimentation procedure involves 5 groups with 12 Wistar albino rats in each group. Rats of Positive cisplatin control receive single injection of cisplatin (7.5mg/kg, Intraperitoneal route) only. The concurrent and pretreatment control rats receive cisplatin on first day and APE (500mg/kg, Oral gavage) on first day and its treatment continue up to 45 days. But the pretreatment control rats receive the APE for 15 days prior to cisplatin treatment. The normal control and extract control rats receive only normal saline and APE respectively during experimental period. The p53 quantification and immunohistochemical analysis were performed on liver samples which were collected from 7^th, 14^th, 28^th and 45^th day of experiment.

The results revealed a significant increase in p53 gene expression in the cisplatin control rats (p<0.05), whereas all APE treated rats showed a significant decrease in p53 expression (p<0.05) as indicated by relative quantification. Immunohistochemical analysis of liver sections demonstrated a higher number of p53-positive cells in the cisplatin control. In contrast, the APE treated control rats showed a marked reduction in both fold expression and the number of cells stained for p53. So, APE may exert anti-apoptotic and antioxidant effects potentially mitigating the cellular damage caused by cisplatin.