Vol. 8, Issue 8, Part R (2024)

Developing cryopreservation protocol for cassava using differential scanning calorimetry and vitrification techniques

Author(s):

M Shankar, GM Puneeth, KB Chethan Kumar, V Thiruvengadam and PL Viswanathan

Abstract:
The present investigation was taken up with the main objective of developing a simple and reproducible cryo-conservation procedure for the important vegetatively propagated cassava crop. Thermogram curves detected by using DSC indicated the formation of ice nucleation at a temperature of -18.4 °C and -18.5 °C in the control shoot apices of YTP1 and Sree Athulya, respectively. In this study, vitrification was performed with nine different vitrification treatments consisting of combination of three factors namely loading solution at different time periods (10 min, 20 min, 30 min) and PVS2 at different time periods (20 min, 30 min, 60 min) and LN2 storage period (24 h, 7 days). The results revealed that irrespective of the genotypes studied, high shoot survival frequency was obtained when the pre-cultured shoot apices were treated in the loading solution for 20 min, dehydrated in PVS2 solution for 40 min and 60 min (LP4 & LP6). SEM analysis showed no marked morphological abnormalities at the cellular level following LS (20 min) and PVS 2 (60 min) treatment. The cryo-treated shoot apices cells appeared as that of the normal untreated shoot apices. However, the negative control shoot apices of cassava (YTP 1) showed major cellular disintegration and shrinkage of cell structures leading to the moderate to heavy cell damage after LN exposure.

Pages: 1413-1420  |  323 Views  105 Downloads

How to cite this article:
M Shankar, GM Puneeth, KB Chethan Kumar, V Thiruvengadam and PL Viswanathan. Developing cryopreservation protocol for cassava using differential scanning calorimetry and vitrification techniques. Int. J. Adv. Biochem. Res. 2024;8(8):1413-1420. DOI: 10.33545/26174693.2024.v8.i8r.2020