Vol. 8, Issue 8, Part P (2024)
Utilizing differential scanning calorimetry for vitrification-based cryopreservation of sugarcane shoot apices
Author(s):
M Shankar, GM Puneeth, KB Chethan Kumar, V Thiruvengadam and PL Viswanathan
Abstract:
Sugarcane (Saccharum sp.) is an important major crop, cultivated largely in tropical and subtropical areas for its high sucrose content. One of the major drawbacks of sugarcane crop in the world is the conventional vegetative propagation leads to difficult in maintaining field gene bank. Cryopreservation is an effective approach to conserve sugarcane germplasm for long term conservation. Thermal behaviour studies using Differential Scanning Calorimetry in both control and cryo treated sugarcane shoot apices were performed to assess the cryo-fitness. The DSC thermogram curves detected the formation of ice nucleation at a temperature of -17.1 °C and -17.2 °C in the control shoot apices of Co 86032 and TN SC Si 7. Nine different vitrification treatments with three factors were assessed for their efficiency in terms of survival of explants cultured on regeneration media after one month. The results revealed that irrespective of the crop and genotypes studied, high shoot survival frequency was obtained when the pre-cultured shoot apices were treated in the loading solution for 20 min, dehydrated in PVS2 solution for 60 min (LP6). SEM analysis showed the absence of any marked morphological variations at the cellular level following LS (20 min) and PVS 2 (60 min) treatment.
Pages: 1250-1257 | 259 Views 99 Downloads
How to cite this article:
M Shankar, GM Puneeth, KB Chethan Kumar, V Thiruvengadam and PL Viswanathan. Utilizing differential scanning calorimetry for vitrification-based cryopreservation of sugarcane shoot apices. Int. J. Adv. Biochem. Res. 2024;8(8):1250-1257. DOI: 10.33545/26174693.2024.v8.i8p.2029