Vol. 8, Issue 8, Part E (2024)

Effect of gamma rays on vegetative growth and biochemical composition of chlorophyll and carotenoid in vM1 generation of chrysanthemum (Dendranthema grandiflora Tzvelve)

Author(s):

Surendra Lal, Ajit Kumar Kapoor, Bhagwan Das Bhuj, Ranjan K Srivastava, Narendra Kumar Singh, Shailesh Chandra Shankhdhar and Ajay Dhyani

Abstract:
Gamma irradiation stimulates a broad range of change in physiology and biochemical alterations in plants by modulating specific defensive and metabolic pathways. Pre-planting cutting irradiation is deliberated as an effective method of increasing production, yield and chemical compositions in plants. In the current study, two varieties of Dendranthema grandiflora Tzelev (Chrysanthemum), namely Lalpari and DFRC-3, were subjected to gamma irradiation with absorbed doses of 10 Gy, 15 Gy, 20 Gy, 25 Gy, 30 Gy, and 35 Gy, along with a control group. Exposure to these varying doses of gamma radiation resulted in consistent changes in the growth and development of the plants. The current study examines the vegetative growth and biochemical composition of chlorophyll and carotenoids in both irradiated and non-irradiated plants of the vM1 generation. The results of the study indicate that gamma irradiation has a significant effect on vegetative growth, chlorophyll and carotenoid content in the vM1 generation of chrysanthemum. The data reveal that vegetative growth, chlorophyll, and carotenoid content decrease significantly in the first generation after gamma irradiation compared to the control group. The highest plant height, number of leaves, and levels of chlorophyll (chl a, chl b, and total chl) and carotenoids were recorded in non-irradiated plants for both varieties. Among the gamma-irradiated plants, those exposed to 10 Gy exhibited the highest vegetative growth, chlorophyll, and carotenoid content, while those exposed to 30 Gy showed the lowest levels in both varieties. This indicates that vegetative growth, chlorophyll, and carotenoid content decrease with increasing gamma doses. Exposure to the higher dose of 35 Gy of gamma rays resulted in plant mortality, with no plants surviving at this dose in both varieties. The biochemical analysis confirms that photosynthetic components are highly responsive to gamma irradiation and serve as a good indicator of persistence, providing substantial evidence of the effects of gamma irradiation. Therefore, gamma rays show promise as a key tool for enhancing breeding efficiency and increasing the frequency of regeneration.

Pages: 327-333  |  387 Views  179 Downloads

How to cite this article:
Surendra Lal, Ajit Kumar Kapoor, Bhagwan Das Bhuj, Ranjan K Srivastava, Narendra Kumar Singh, Shailesh Chandra Shankhdhar and Ajay Dhyani. Effect of gamma rays on vegetative growth and biochemical composition of chlorophyll and carotenoid in vM1 generation of chrysanthemum (Dendranthema grandiflora Tzvelve). Int. J. Adv. Biochem. Res. 2024;8(8):327-333. DOI: 10.33545/26174693.2024.v8.i8e.1750