Vol. 8, Issue 6, Part G (2024)

Abstract: Nanjanagud Rasabale (AAB) is a well-known mostly farmed cultivar that has been given a GI (Geographical Indication) tag for its provenance, distinctive flavour, aroma, colour, fibrous texture and nutritional value. Due to its great susceptibility to the soil-borne panama wilt disease, which is brought on by Fusarium oxysporum f.sp. cubense, this leading cultivar is currently failing to maintain its GI designation. The present investigation was carried out on in vitro conservation by media manipulation using osmotic agents through slow growth culture technique. Shoot tip explants were cultured on MS basal medium supplemented with osmotic agents (Mannitol, Sorbitol, Sucrose and Maltose). Among different osmotic agents treated media effective storage (145.66 days) was recorded in sorbitol 35 g/L. Determination of genetic stability was performed by assessment of Inter Simple Sequence Repeat amplification (ISSR). All the 15 primers amplified unambiguous, readable and showed monomorphic bands. The cultures were conserved by extending the subculture period and prolonged storage period without any genetic variation by using osmotic agents.