Vol. 8, Issue 6, Part E (2024)

Abstract: In India the awareness about genetic disorders is too less among famers at field level, reproductive efficiency is a critical component of growing dairy, but reproductive insufficiency is highly economic problems today. Among that all reproductive problems, anoestrous and repeat breeding are major seen at field by many veterinarians, so it is important and needful to research and resolve the issues regarding to avoid farmers economic loss. There is possibility of mutant genes that are directly affect to livestock reproductive performance. With that concerned it is essential to screen the animals to avoid the increase disease prevalence. Present investigation undertaken, cows with anoestrous and repeat breeding to identify Complex Vertebral Malformation (CVM) and Uridine -5 Monophosphate Synthase (UMPS) molecular genetic disorders by Polymerase Chain Reaction (PCR) - Restriction Fragment Length Polymorphism (RFLP). For this study, Blood of 50 Anoestrous and 52 Repeat Breeding cows (102) were collected from Maharashtra state, India. The de-oxy ribose nucleic acid (DNA) has extracted, and PCR amplification was performed for SLC35A3 and UMPS genes at respective annealing temperature, shown 287 and 108 bp bands respectively. PCR product digested with restriction enzymes PST I and Ava I at 37 °C for 4 hours for CVM and DUMPS respectively. The results of CVM and DUMPS shown 264 & 23 bp bands and 53, 36 and 19 bp bands, respectively. But due to modest size, 19 bp has not visible in RFLP which is seen in wild animals. All selected animals had shown normal results for CVM and DUMPS.