Vol. 8, Issue 6, Part C (2024)

Abstract: Plant Growth Promoting Rhizobacteria (PGPR) are recognized as vital biological agents for enhancing plant growth, with Actinomycetes being a prominent group among them. The study aimed to isolate and screen diversity of actinomycetes from replant site of apple rhizosphere as growth promoter of apple in vitro. Six actinomycetes isolates have been cultured from apple rhizosphere on Kenknight media. All the actinomycetes isolates underwent screening for plant growth promoting activitites including Phosphate solubilization, siderophores, ammonia, HCN and Protease production. Based on their superior plant growth-promoting attributes, the A3 isolate was prioritized for further analysis via 16S rRNA sequencing. The analysis revealed a high degree of homology between the A3 isolate and Streptomyces bluensis (with a similarity of ≥98%). Our finding underscore a potent A3 actinomycetes isolate as a beneficial contributor to addressing replant issues in apple cultivation and promoting sustainable agricultural practices..