International Journal of Advanced Biochemistry Research

Biochem
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Vol. 8, Issue 5, Part B (2024)

Molecular characterization of bell pepper (Capsicum annuum L.) infecting Capsicum chlorosis orthotospovirus

Author(s): Dr. M Lakshmi Naga Nandini, Dr. CH Ruth and Dr. M Ravindra Babu
Abstract: In 2023, surveys were carried out in key bell pepper cultivation regions of the Andhra Pradesh district to gather virus isolates for analysis. The virus responsible was identified based on its symptoms, how it spreads, and through serological and molecular investigations. Affected plants exhibited stunted growth, dwarfism, and concentric chlorotic ringspot symptoms on their leaves. Infected bell pepper fruits showed deformities with nailhead symptoms, or ripe fruits displayed pale yellow ringspots of various colours. Samples showing characteristic symptoms were gathered and employed to inoculate healthy bell pepper plants. Serological testing using Tospo (I, II, III) and CaCV antisera via DAS-ELISA identified the virus as Capsicum chlorosis orthotospovirus (CaCV), as it reacted solely with CaCV antisera. Further molecular analysis, including RT-PCR, confirmed the virus's identity as Capsicum chlorosis orthotospovirus.
Pages: 112-119  |  63 Views  24 Downloads


International Journal of Advanced Biochemistry Research
How to cite this article:
Dr. M Lakshmi Naga Nandini, Dr. CH Ruth, Dr. M Ravindra Babu. Molecular characterization of bell pepper (Capsicum annuum L.) infecting Capsicum chlorosis orthotospovirus. Int J Adv Biochem Res 2024;8(5):112-119. DOI: 10.33545/26174693.2024.v8.i5b.1063
International Journal of Advanced Biochemistry Research
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