Vol. 8, Issue 5, Part L (2024)

Chilli (Capsicum annuum L.) is one of the most important vegetable crops grown throughout the globe. It is prone to viral infections under field conditions. Among the viruses; Cucumber mosaic virus is severely affecting the production and productivity of Chilli. Cucumber mosaic virus is most devastating plant pathogen infecting more than 1200 species across 100 families of monocot and dicot plants. In the current investigation we have conducted field survey for the identification of the CMV. During the field survey in the Bhagalpur district of Bihar, we were collected 95 chilli leaves samples showing characteristics symptom of CMV, such as mild mosaic, mottling and leaf distortion. DAC-ELISA was performed for detection and screening of the CMV. The observation that the chilli samples exhibited CMV symptoms from Sabour and Kahalgaon block, accounting for 25.71% and 16.66% of the ELISA findings against CMV antiserum, was surprising. The highest incidence of CMV was recorded in sabour block i.e. 25.71% and lowest incidence was recorded in Kahalgaon block i.e. 16.66%. After confirmation with the DAC-ELISA, single lesion assay was performed for the purification of the CMV virus from other contaminants. Identified CMV strain was biologically characterized by mechanically transmissible to the host plant. Seven days after the inoculation, we saw chlorotic local lesions on Chenopodium amaranticolar. It is comparable to several CMV research published by different scientific groups