Author(s): AA Suryawanshi, DA Pawalkar, RV Gaikwad, CN Galdhar and JU Patil

Abstract: 103 cross bred cattle selected randomly irrespective of age, breed, sex, physiological status, farming system etc. and blood samples were collected in EDTA and plain vials from Khandala, Phaltan, Lonand and Pandharpur taluka of Satara and Solapur district. 3/103 (2.92%) animals were positive for T. annulata by PCR while in Satara district 3/44 (6.81%). 78/103 (75.72%) animals were positive for T. orientalis by PCR while in Satara district 32/44 (72.72%) and Solapur 46/59 (77.96%). 2/103 (1.94%) animals found positive by PCR for mix infection of T. annulata and T. orientalis. Haemato-biochemical findings of T. annulata PCR positive and T. annulata PCR negative animals have significantly higher PCV (P<0.01) and TEC (P<0.01), while statistically significant (P<0.01) monocytosis was observed in T. annulata PCR positive animals. In biochemical findings higher values of alkaline phosphatase and aspartate transaminase in T. annulata PCR positive animals were statistically significant (P<0.05), and similarly in T. annulata PCR negative animals higher values of alanine transaminase and total proteins were statistically significant (P<0.05). Haemato-biochemical findings of T. orientalis PCR positive with T. orientalis PCR negative. T. orientalis negative animals neutrophils and lymphocytes were higher and the difference was statistically significant (P<0.05), while T. orientalis PCR positive animals had lower monocytes (P<0.01). The biochemical findings of T. orientalis PCR positive with T. orientalis PCR negative animals were statistically non-significant.

DOI: 10.33545/26174693.2024.v8.i4Sg.1077