International Journal of Advanced Biochemistry Research

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Vol. 8, Special Issue 4, Part D (2024)

Isolation and histopathological examination of P. multocida in pneumonic cases of Buffalo

Author(s): Neeteesh Kumar, D Niyogi, Dharam Prakash Shrivastava, Arunima Singh, Akshit Tyagi, KK Tripathi and Vibha Yadav
Abstract: A total 180 cases of buffaloes slaughtered in the slaughter house of Unnao and Barabanki district of Eastern Uttar Pradesh was surveyed during the month October, 2022 to March, 2023. The lungs exhibiting gross pathological lesions was put into 10% neutral formalin for histopathological studies and other part of lung for bacteriological studies. On Gross examination maximum cases of congestion and hemorrhages in lung were observed followed by emphysematous conditions and pulmonary oedema in the slaughtered animal in both the districts. Microscopic sections of lungs were examined and the major histologic findings are vascular and bronchiolar congestion along with severe emphysema seen in some cases. Some of the Emphysematous lung showed hyperplasia of the bronchiolar wall and peribronchial lymphoid tissue and exudates in the alveoli. In the bacteriological study, a total samples which showed gross lesions in slaughtered buffaloes in both the districts were collected. Among the tested samples (34 and 26 samples from Unnao and Barabanki districts respectively), were identified as Pasteurella spp. depending on their cultural/colony characters, morphology and biochemical properties viz Indole, Methyl red, Catalase production, Urea hydrolysis and Citrate utilization. Out of 34 isolates, 32 showed positive results for Indole test, 24 positive for catalase, 24 isolates, were positive for Urea hydrolysis, 23 isolates considered as positive for Methyle red. All isolates tested negative for gelatin liquefaction but 30 isolates were found positive for citrate utilization. Hence, so many unhealthy/diseased buffaloes are being slaughtered in the slaughtered houses for human consumption and that meat should not be consumed or may be condemned as not fit for human consumption due to infections.
Pages: 268-273  |  91 Views  29 Downloads


International Journal of Advanced Biochemistry Research
How to cite this article:
Neeteesh Kumar, D Niyogi, Dharam Prakash Shrivastava, Arunima Singh, Akshit Tyagi, KK Tripathi, Vibha Yadav. Isolation and histopathological examination of P. multocida in pneumonic cases of Buffalo. Int J Adv Biochem Res 2024;8(4S):268-273. DOI: 10.33545/26174693.2024.v8.i4Sd.959
International Journal of Advanced Biochemistry Research
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