International Journal of Advanced Biochemistry Research

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Vol. 8, Special Issue 4, Part C (2024)

Effect of black cumin (Nigella sativa) seeds extract in tris egg yolk citrate extender on viability of cryopreserved Surti buck semen

Author(s): AS Kardani, LC Modi, NF Chaudhari, AA Pathan, HC Sharma and DB Bhoi
Abstract: The present research work was conducted to examine effect of different concentrations of Black cumin (Nigella sativa) seeds extract in Tris egg yolk citrate extender on sperm viability of cryopreserved Surti buck semen. Total 64 semen ejaculates were collected from four Surti buck (16 ejaculates/buck) twice a week by artificial vagina method. Semen samples were diluted with Tris egg yolk citrate extender and Black cumin (Nigella sativa) seeds aqueous extract was supplemented at different concentrations viz. 0% (T1), 3% (T2), 4% (T3), 5% (T4) (pH 6.5-6.8) maintaining final concentration of 100×106 sperm/ml. After making the groups, the semen samples were examined for structural plasma membrane integrity (viability) (%) and functional plasma membrane integrity (HOST) (%) for all groups. Result showed that highest mean live sperm (%) and mean host reacted sperm (%) was found at initial, pre-freeze and post-thaw stage in T3 group followed by T2, T4 and T1 group. It was concluded that post-thawed viability and host reacted sperm (%) parameters were well maintained at 4% supplementation of Black cumin (Nigella sativa) seed extract in Tris egg yolk citrate extender.
Pages: 183-187  |  205 Views  76 Downloads


International Journal of Advanced Biochemistry Research
How to cite this article:
AS Kardani, LC Modi, NF Chaudhari, AA Pathan, HC Sharma, DB Bhoi. Effect of black cumin (Nigella sativa) seeds extract in tris egg yolk citrate extender on viability of cryopreserved Surti buck semen. Int J Adv Biochem Res 2024;8(4S):183-187. DOI: 10.33545/26174693.2024.v8.i4Sc.943
International Journal of Advanced Biochemistry Research
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