Author(s): Vikas Singh, Mukesh Kumar, Rakesh Singh Sengar, Vaishali, Lokesh Kumar Gangwar and Ramji Singh

Abstract: Two sugarcane cultivars (COLK 14201 and CO 15023) were used to induce callus, and the response of various Plant Growth Regulators (PGR) to this process was assessed. Different concentrations were selected for the best callus induction after optimization. The combination of 2,4-D alone and in combination with the other growth regulators (BAP and kinetin) was used for callus culture. The best callus response in the terms of number of days, callus fresh weight and callus frequency in COLK 14201 was observed on MS media provided with 2,4-D (4 mg L^-1) while in CO 15023 the best response was on MS + 2,4-D (3 mg L^-1) + Kinetin (0.5 mg L^-1). The maximum callus frequency was observed 100 percent in 2,4-D (3 mg L^-1) but callus was not good than 2,4-D (4 mg L^-1). Other parameters were also recorded like callus texture and callus color.

DOI: 10.33545/26174693.2024.v8.i4h.1022