Author(s): Doaa H Alasady, Rawaa SA AL-Azawi, Hamzah H Kzar, Noora H Ali and Zainab A Radhi

Abstract: One of the main complications of diabetic mellitus (DM) is diabetic foot ulcer (DFU) which leads to significant morbidity and mortality among affected individuals worldwide. This study was designed to assessment of DFU with some of the biochemical markers and genetic variants, in Iraqi patients with DM. The current study involved 135 subjects that were subdivided into 45 patients with (DFU), 45 diabetics without (NDFU), and 45 subjects as controls (CONT). The genotyping analysis using restriction enzymes HaeIII and NcoI (PCR-RFLP) for estimation of genotypes of VEGF (rs3025039) and TNF-α (rs1800629) and the results reveal the association with increased susceptibility to DFU in diabetic patients. The results of this study showing statistical differences in the biochemical markers, HbA1c and CRP in the DFU group compared to both NDFU and CONT group. On the other hand, the genetic analyses showed significant differences in the SNP of VEGF and TNF-α genotypes between the DFU group and CONT group. Odds ratios were calculated for each genotype to investigate the genetic polymorphism differences in all study groups. In conclusion, the results show the main role of VEGF (rs3025039) and TNF-α (rs1800629) gene polymorphism in the incidence of DFU in Iraqi population.

DOI: 10.33545/26174693.2024.v8.i4f.989