Author(s): A Jayasri, P Eswara Prasad, BDP Kala Kumar, K Padmaja, M Sonali and Dr. E Kumar

Abstract: Bovine mastitis is a prevalent inflammatory disease in dairy industry which causes reduced milk yield. It is mainly due to microbial infections involving various bacteria. Among them Staphylococcus aureus is the most common pathogenic agent whose resistance to antibiotics have led to difficulty in the treatment of mastitis in cattle. However, recent studies have demonstrated the antimicrobial effect of metal-based nanoparticles which can be applied for treating multidrug resistant bacterial infections. Therefore, the present study was undertaken to study the effect of metal-based nanoparticles viz Silver and zinc oxide nanoparticles which were biologically synthesised from the leaves of Thespesia populnea in the treatment of Staphylococcus aureus induced mastitis in mice by studying the variations in haematological indices. A total of 48 mice were equally divided into six groups where group I served as control group while remaining groups were induced with mastitis. Groups III, IV, V and VI were treated with Thespesia populnea leaf extract, T. populnea methanolic extract mediated nano silver (TPNS), T. populnea methanolic extract mediated nano ZnO (TPNZ) and Ceftriaxone respectively. The blood parameters like haemoglobin, RBC, PCV, MCV, MCHC, WBC, Neutrophils, Lymphocytes, Esinophils and Monocytes were studied. The abnormal deviation of these parameters when compared with control group indicated induction of mastitis. However, in the treated groups restoration of the above blood parameters was seen in the increasing order of III, V, VI and IV. From the results, it can be inferred that silver and zinc oxide nanoparticles have more effect in the restoration of the haemotological values when compared with Thespesia populnea leaf extract alone due to the cumulative effect.

DOI: 10.33545/26174693.2024.v8.i4d.974