Author(s): Bhagchand Chhaba, Ediga Arun Goud, Sushree Akankshya Das, Suman Karmakar, Sangram Keshari Rout and Vikram C Kachave

Abstract: The efficacy of Ocimum sanctum (Tulsi) and Tagetes erecta (Marigold) on growth-promotion, haematological and biochemical profiles in fingerlings of Labeo rohita was evaluated. Different diets were prepared 0% (control), 1% Tulsi (T₁), 3% Tulsi (T₂), 1% Marigold (T₃) and 3% Marigold (T₄) and fed to the fishes. The sampling of the experiment was done on the 18^th, 36^th, 54^th, 72^th and 90^th days for growth, haematological, biochemical parameters and water quality parameters analysis. The lowest FCR and significantly higher SGR, FCE, and percentage (%) weight gain were found in T₂ group. Highest body weight gain, length and percentage length was found in T₄ group. At the end of the experiment the biochemical parameters indicated that inclusion of Ocimum sanctum and Tagetes erecta in feed significantly (p<0.05) increased the serum total protein, albumin and globulin content. Results revealed that administration of Ocimum sanctum and Tagetes erecta through different diets mainly in T₂, T₃ and T₄ diets increased the total erythrocyte count, haemoglobin and total leukocyte count which enhanced the innate immune response. The physico-chemical parameters to be analysed include pH, temperature, total alkalinity, ammonia–nitrogen, dissolved oxygen and phosphate-phosphorus. The result of the present study indicated that all parameters are optimum for fish culture. The incorporation of Tulsi and Marigold extract @ 3% in the feed may influence the innate immunity, growth performance and resistance of fish.

DOI: 10.33545/26174693.2024.v8.i4b.971