Vol. 8, Issue 4, Part b (2024)
Evaluation secondary metabolite extract produced by Aspergillus terreus isolated from poultry droppings as antimicrobial agent
Author(s):
Sanaa A Ghali, Furdos N Jafer and Areej HS Aldhaher
Abstract:
The purpose of this study: was to separate fungi that produce antibiotics from bird droppings.
Place and Time of Study: Between October 2022 and March 2023, poultry droppings were gathered from farms and marketplaces in Poultry fields in Basra / Karma Ali.
Approach: A total of 150 samples of bird droppings were gathered. Subsequently, 1 gram of freshly deposited chicken droppings were measured and diluted in a sequential manner. The resulting dilutions were then cultivated for a duration of five days on malt extract agar and potato dextrose agar. Microscopical and morphological description of Aspergillus terreus. The mushroom extract's minimum inhibitory concentration (MIC) against pathogenic bacteria was determined using the agar well diffusion technique. The extract's aggressive behavior was evaluated against a total of four bacterial isolates, encompassing both Gram-positive and Gram-negative strains.
Results: The results showed that the fungal extract isolated from Aspergillus terreus showed aggressive behavior against pathogenic bacteria, as extracts of Aspergillus terreus secondary metabolites were effective against Klebsiella pneumonia, Bacillus cereus, Staphylococcus aureus, and Escherichia coli in a range of doses where the inhibitory concentration was the lowest for Escherichia coli and Staphylococcus aureus is 15 mg. /ml, while the minimum inhibitory concentration for Bacillus cereus and Klebsiella pneumonia was 33.5. When the extracts are refined, they can be used to create new medicinal molecules from natural sources.
Pages: 144-152 | 579 Views 239 Downloads
How to cite this article:
Sanaa A Ghali, Furdos N Jafer and Areej HS Aldhaher. Evaluation secondary metabolite extract produced by Aspergillus terreus isolated from poultry droppings as antimicrobial agent. Int. J. Adv. Biochem. Res. 2024;8(4):144-152. DOI: 10.33545/26174693.2024.v8.i4b.940