The Molecular experiment is put out inthe biotechnology laboratory of department of genetics and plant breeding at College of Agriculture, S. D. Agricultural University, Sardarkrushinagar. A total of seventeen SSR primers were used for the study of thirty genotypes, of which ten primers showed amplification, among ten primers six were polymorphic amplifying viz. barc 186, CFA 2114, GWM 148, CFD 71, Xgwm 11, Xgwm484. The PIC values for these six primers ranged from 0.14 (Xgwm484) to 0.36 (CFA 2114) with an average of 0.29. The PIC values provide information about amount of variability for a primer among the genotypes studied. The dendrogram generated through sub-programme cluster tree analysis of the NTSPYC-pc software divided thirty genotypes into two major clusters A and B. Cluster A is subdivided into two sub clusters A1 and A2. Sub cluster A1 includes one genotype VA2020-15 whereas sub cluster A2 consists of one genotype VA2020-06. Similarly, Cluster B is subdivided into four sub clusters i.e., B1, B2, B3 and B4. Sub cluster B1 consists of one genotype VA2019-06, likewise sub cluster B2 also included three genotypes, B3 consist fifteen genotypes and B4 consist nine genotypes.

These primers are use in further molecular breeding programmes for improving the ability of particular trait and the information are important for future as gene cloning and also provide support for the implementation of marker-assisted selection (MAS).