Trial was sown at Main Cotton Research Station, Navsari Agriculture University, Surat during 2021-22 under protected and unprotected condition for sucking pest. Ten cotton genotypes were selected for study with 3 replication RBD design. All agronomical practices and plant protection measures were adopted as and when required. Biochemical parameters were perform at 90 DAS using standard methods. Yield and yield attributing parameters were observed at harvest. The plant height was significant higher due to protected condition as compare to unprotected condition. The genotypes GISV-236 (153 cm) showed significant plant height which was at par with GISV-366 (152 cm). No of sympodia showed significant decrease due to unprotected condition GSHV-191 (1049.1 kg/ha) relieved significant higher seed cotton yield followed by GISV-375 (1041.5 kg/ha), GISV-382 (942.7 kg/ha) and GISV-236 (911.8 kg/ha) respectively. Protein was found highest in GISV-375 (18.36 mg/g) under unprotected environment for Jassid while sugar was found higher in GISV-371 under unprotected compared to protected condition. Phenol content was observed highest in GSHV-191 (40.12 mg/g) under protected field while it GISV-323 (41.62 mg/g) showed highest under unprotected condition. Range of tannin content 24.07 to 34.97 (mg/100g) was observed. Non-bt genotypes under both condition showed significant difference for flavonoid and gossypol content. GISV-366 under (0.83%) under protected and unprotected condition recorded higher content of gossypol while GISV-236 showed lower under protected condition.