Vol. 8, Issue 3, Part E (2024)

The prevalence of E. coli in raw milk and their susceptibility to antibiotics

Author(s):

Himani Prajapati and RD Yadav

Abstract:
Escherichia coli, sometimes known as E. coli, is a beneficial member of the natural symbiotic bacterial flora found in the stomach. One of the main causes of bovine mastitis, which can range from a serious systemic illness to a subclinical mammary gland infection, is E. coli. Most commensals are benign, but some have developed virulence features that can spread through contaminated milk and infect otherwise healthy people. Antibiotic-resistant E. coli found in raw cow's milk may be the cause of resistance illnesses in people. The study looks into the antibiotic susceptibility of E. coli and how often it is in raw milk samples from dairy farms in Haryana.
For this cross-sectional investigation, 40 raw cow milk samples were gathered from different areas in Haryana. Following the presumed isolation of E. coli from raw milk, a two-stage NDRI assay was conducted to confirm the isolates of E. coli utilising quick procedures. The IMViC assays were used to biochemically characterise isolates of E. coli samples. Using the Kirby-Bauer method, antibiotic susceptibility was assessed for third- and fourth-generation cephalosporins.
The NDRI test verified that 15% of the 40 raw milk samples had E. coli germs in them. Every E. coli sample with phenotypic confirmation was susceptible to carbapenem and broad spectrum cephalosporin drugs.
15% of raw milk from Haryana's dairy had E. coli, although the bacteria did not show signs of drug resistance to cephalosporins.

Pages: 406-410  |  409 Views  187 Downloads

How to cite this article:
Himani Prajapati and RD Yadav. The prevalence of E. coli in raw milk and their susceptibility to antibiotics. Int. J. Adv. Biochem. Res. 2024;8(3):406-410. DOI: 10.33545/26174693.2024.v8.i3e.758