Author(s): Santosh Sawardekar and Sandip Sherkar

Abstract: Aglaonema, a popular ornamental plant valued for its vibrant foliage and aesthetic appeal. This study aimed to establish an efficient protocol for in vitro propagation of Aglaonema variety Red valentine using tissue culture techniques. The experiment involved the establishment of sterile cultures from nodal explants. Murashige and Skoog (MS) medium supplemented with various concentrations of plant growth regulators used to stimulate organogenesis. Surface sterilization of explants with carbendazim (0.2%) + Plantomycin (200 mg/l) for 30 minutes, along with Ethanol 70% for 1 minute, Sodium hypochlorite 10% for 15 minute, and Mercuric chloride 0.3% for 15 minute, showed the highest aseptic cultures (88.67%). Murashige and Skoog’s medium supplemented with 3.0 mg/l Benzyl-amino-purine resulted in 84% of bud sprouting. Highest multiplication ratio (1: 2.53) obtained on Murashige and Skoog’s medium supplemented with 6 mg/l Benzyl-amino-purine and 2 mg/l of Kinetin. Maximum rooting (95%) was observed in medium supplemented with 2.0 mg/l Indole-3-butyric acid and 0.5 mg/l Naphthalene acetic acid. Plantlets were successfully hardened in 100% of Cocopeat in greenhouse.

DOI: 10.33545/26174693.2024.v8.i2h.821