Author(s): DJ Wankhade, Jadhav PK, Deshmukh AS, Gholave KK, VR Hinge and Chavan RL

Abstract: In this study, we explored the In vitro shoot regeneration potential of pigeon pea (Cajanus cajan) cv. BSMR 853 through two distinct pathways: organogenesis and somatic embryogenesis. The use of different plant growth regulators and culture conditions was investigated to optimize shoot regeneration efficiency. For organogenesis, embryos from 5-day-old germinated seeds were employed, and we observed that the highest shoot bud regeneration occurred on media supplemented with 2 mg/L 6-benzylaminopurine (BAP). Subsequent elongation of shoots was achieved by reducing the BAP concentration. In contrast, somatic embryogenesis was initiated from embryo of 5-day-old germinated seeds. Here thidiazuron (TDZ) was used to instead of BAP as a growth regulator. The development of globular embryos was rapid, and the embryos were prolific with TDZ. These globular embryos were then matured using MS media supplemented with abscisic acid (ABA) and subsequently germinated on half-strength MS media supplemented with lower concentrations of BAP. Comparative analysis of the two regeneration pathways in pigeon pea cv. BSMR 853 revealed that despite somatic embryogenesis producing a greater number of embryos, organogenesis led to a greater success rate in establishing shoots. This study provides valuable insights into the regeneration processes of pigeon pea, offering a basis for further research and applications in crop improvement and biotechnology.

DOI: 10.33545/26174693.2024.v8.i2f.597