Author(s): Bharsakale RD, Wankhade DJ, Jadhav PK, Gholave KK, Deshmukh AS, Chavan RL and VR Hinge

Abstract: The Begomoviruses genus' Mungbean Yellow Mosaic Virus (MYMV) causes considerable annual yield losses in crops like soybean, mungbean, cowpea, and black gram, amounting to about $300 million. This study provides proof that symptomatic samples taken from different parts of Maharashtra include MYMV infection. The gene-specific amplification of the CP gene (719 bp and 387 bp) served as the basis for the confirmation. Its identity as MYMV was confirmed by the astonishing 96.52% similarity between the MYMV coat protein (AV1) gene sequence (Accession No. JQ004982.1) and the CP gene sequence found in cowpea samples from the Pune region. A BLASTp investigation of the coat protein of the Mungbean Yellow Mosaic Virus in Vigna (Accession No. AAW50913.1) indicated a 93.41% similarity. With an identity of 82.99%, the MYMV strain that infected cowpea in the Pune area showed the most genetic divergence from Indian strains (MYMIV and HYMV) and a close genetic relationship (96.52%) with MYMV. The 70 different CP gene sequences of MYMV that were analyzed phylogenetically clustered into three groups based on nucleotide and protein sequences. Four sequences (45%), including the MYMV Pune isolate, showed the highest pairwise identity (96-98%) out of the 70 sequences analyzed, whereas about 25% of the total sequences showed lower pairwise identities (84-87%). Significant differences in restriction sites were found among the selected CP gene sequences under examination after an in silico restriction analysis was conducted by using the CisSERS tool. The MYMV strain's broad distribution in Maharashtra, its ability to infect other legume crops, and its ongoing evolution with the Mungbean Yellow Mosaic India Virus (MYMIV) strain are all highlighted by this study.

DOI: 10.33545/26174693.2024.v8.i2f.596