International Journal of Advanced Biochemistry Research

Biochem
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Vol. 8, Special Issue 1, Part J (2024)

Detection of hla and hlb gene in Staphylococcus aureus isolates from buffaloes with subclinical mastitis

Author(s): Pankaj Dhakarwal, Shivendra Kumar Bhalothia, Veenu Singhal, Dilip Singh Meena, Hukma Ram Parihar and Anil Kumar Kataria
Abstract: Staphylococcus aureus (S. aureus) is a spherical, non-motile, gram-positive non-spore forming bacterium which plays a critical role to cause subclinical mastitis in buffaloes. In present study 84 milk samples were collected from Hanumangarh city, 41 S. aureus isolate were isolated with a recovery of 48.80%. All 41 samples were genotypically confirmed by 23S rRNA based ribotyping method. All the 41 isolates were typable for haemolysin genes where hla gene was detected in 37 (90.24%) and hlb gene in 34 (82.92%) isolates. On sheep blood agar 25 isolates (60.97%) produced complete hemolysis, four (9.75%) incomplete hemolysis both complete and incomplete by four (9.75%) while eight (19.51%) isolates did not produce any hemolysis. Hence S. aureus isolates obtained from buffaloes with subclinical mastitis showed higher percentage of haemolysin both genotypically and phenotypically.
Pages: 738-742  |  299 Views  112 Downloads


International Journal of Advanced Biochemistry Research
How to cite this article:
Pankaj Dhakarwal, Shivendra Kumar Bhalothia, Veenu Singhal, Dilip Singh Meena, Hukma Ram Parihar, Anil Kumar Kataria. Detection of hla and hlb gene in Staphylococcus aureus isolates from buffaloes with subclinical mastitis. Int J Adv Biochem Res 2024;8(1S):738-742. DOI: 10.33545/26174693.2024.v8.i1Sj.417
International Journal of Advanced Biochemistry Research
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