Antimicrobial drug resistance profiling of Pseudomonas aeruginosa isolated from nosocomial infections
Author(s): Rekha Panwar, Priyanka Kumari and Basant
Abstract: As the second most important opportunistic pathogen, P. aeruginosa is thought to account for 10–11% of all nosocomial infections. It is associated with several nosocomial infections, such as burns and wounds, pneumonia, urinary tract infections, and cystic fibrosis. Out of 65 samples, ten P. aeruginosa isolates were recovered from different hospital-acquired infections in different species. After molecular-level conformation, the antimicrobial sensitivity of 23 drugs against all isolates was evaluated by performing a Cutler sensitivity test. Polymyxin-B, gentamicin, ceftazidime, meropenem, carbapenems, and ticarcillin were found to be 100% effective against all P. aeruginosa isolates. Aminoglycosides (i.e., amikacin and kanamycin) showed susceptibility to 80% of the isolates. Only half of the isolates exhibited sensitivity to chloramphenicol. Whereas cefuroxime, cefotaxime, amoxicillin, ampicillin, vancomycin, tetracycline, clindamycin, erythromycin, and trimethoprim exhibited 100% resistance. In conclusion, all isolates of P. aeruginosa recovered from nosocomial infections showed evidence of multidrug resistance. Out of 23 tested antibiotics, the P. aeruginosa isolates exhibited either a complete or partial drug resistance phenotype against 17 antimicrobal agents, which eventually can cause prolonged persistence infections and treatment failure.
