Abstract: This study at PGIVER, Jaipur, investigated canine mast cell tumors, prevalent in veterinary oncology, comprising 20% of cutaneous tumors. Employing FNAC and impression smears for cytological diagnosis, 58 samples from diverse breeds revealed a 6.90% incidence. Mast cell tumors exhibited an irregular morphology and firm consistency. Cytologically, smears showed heightened cellularity with round neoplastic cells, anisocytosis, and metachromatic granules. Histopathologically, pleomorphic mast cells with metachromatic granules were observed. The study highlights the correlation between cytology and histopathology, suggesting routine cytological use for neoplastic disease diagnosis.