The primary objective of this investigation was to discern variations in biochemical parameters associated with diarrhea in cattle calves across different seasons, specifically the rainy, autumn, and winter periods. A total of 90 diarrheic cattle calves, with 30 representing each season, were meticulously chosen based on their diarrheic condition. The biochemical profile of the diarrheic calves revealed noteworthy distinctions among the seasons. During the rainy season, levels of ALT, AST, total protein, glucose, magnesium, and phosphorus were markedly higher (p<0.05) compared to the autumn and winter seasons. Conversely, levels of albumin, urea, and creatinine exhibited a significant increase (p<0.05) in the winter season when juxtaposed with those in the autumn season. Notably, cholesterol and calcium levels demonstrated a comparable pattern across all three seasons. The study underscores the intricate interplay between environmental conditions, diarrheal incidents, and the physiological responses of calves in a gaushala. The observed variations in blood serum parameters provide valuable insights for understanding and managing the health of cattle in such settings. These findings emphasize the importance of considering seasonal factors and implementing targeted interventions for effective herd management and health maintenance within a gaushala environment.