Biodiversity assessment plays a crucial role in understanding the composition and distribution of plant species within a specific area. This research paper aims to assess the biodiversity in homegarden agroforestry system of the Yeshu Darbar area in SHUATS Campus, Prayagraj during the rainy season. Prayagraj is situated at an elevation of 78 meters above sea level at 25.87 North latitude and 81.15° E longitudes. This region has a sub-tropical climate prevailing in the South-East part of U.P. with both the extremes in temperature, i.e., the winter and the summer. In cold winters, the temperature sometimes is low as 32°F in December-January and very hot summer with temperature reaching up to 115°F in the months of May and June. Frost during winter and hot scorching winds during summer are also common. The average rainfall is around 1013.4 (cm) with maximum concentration during July to September months with occasional showers in winters. The phyto-sociological analysis was conducted to study the composition and distribution of trees and herbs in Yeshu Darbar Area home gardens during the rainy season. The study focused on three different areas: North, South and East. The data collected included the density, frequency, abundance, relative density (RD), relative frequency (RF), relative dominance (RDO), and importance value index (IVI) of each species. Among all areas from the Phyto sociological structure of Tree Species and herb species The total number of each individual species found in Yeshu Darbar north Trees (14), Herbs (12), Yeshu Darbar South Trees (12), Herbs (12) species and in Yeshu Darbar East area Trees (12), Herbs (15), are found in total Yeshu Darbar area of SHUATS Campus. In the North home gardens, Psidium guajava and Mangifera indica were the dominant tree species, with IVIs of 32.14 and 45.48, respectively. Among the herbs, Coriandrum sativum and Asarum canadense had the highest IVIs of 24.24 and 22.40, respectively. In the South home gardens, Mangifera indica and Callistemon were the dominant tree species, with IVIs of 55.19 and 38.49, respectively. Among the herbs, Trigonella foenum-graecum and Cynodon dactylon had the highest IVIs of 28.79 and 38.90, respectively. In the East home gardens, Cestrum nocturnum and Psidium guajava were the dominant tree species, with IVIs of 43.58 and 33.36, respectively. Among the herbs, Abelmoschus esculentus and Solanum lycopersicum had the highest IVIs of 32.89 and 42.25, respectively. The study provides valuable insights into the composition and distribution of trees and herbs in the Yeshu Darbar Area home gardens during the rainy season. It highlights the importance of certain species in each area and can serve as a basis for future research and conservation efforts in the region.