Cancer is a disease of unlimited cellular growth and division which lead to the disturbance of cellular homeostasis, destruction to the immune system, and different impairment that can be catastrophic to the cells. Chronic inflammation is widely recognized as a major risk factor for cancer formation, but the underlying mechanisms are poorly understood. Recently, it was shown that Gasdermin D (GSDMD) protein drives pyroptotic cell death in macrophages on cleavage by inflammatory caspases Allow massive release of pro-inflammatory mediators. In this study, we examined the role of B.hydroxy butyrate as a regulator of cell death pathway in Ehrlich’s Ascites Carcinoma (EAC).50 female albino mice randomly divided into five equal groups:Group N:negative control, Group E (Ehrlich ascetic bearing mice), Group E+K (EAC- bearing group co-treated with ketone bodies, Group K+E+K (ketone bodies pretreated group), GroupK:ketone bodies control group.Ascetic cells and mammary gland tissue were homogenized and used for the assay of the gasdermin D level by ELISA. Keto diet significantly increase trypan blue positive dead cells. This was associated with decreased gasdermin D in all treated groups when compared to the untreated group.

Conclusion: The current study obscure that inflammatory cell death pathways pyroptosis which is one of key element of tumor microenvironment was significantly reduced through keto diet administration.