In present investigation, for the development of Fusarium tolerant varieties in carnation, highly susceptible cultivars viz. Tempo and Raggio de Sole were used. Calli of caraation cultivars were irradiated to different doses of gamma irradiation. And Callus surviving at highest dose of gamma rays was mass multiplied on callus multiplication medium (MS medium +10 μNAA + 10 μM kin). Irridiated calli after multiplication, was treated with different concentrations of culture filtrate of Fusarium oxysporum f.sp. dianthi. 20% Culture resulted Fusarium wilt insensitive mutants at the end of 3rd selection cycle.