International Journal of Advanced Biochemistry Research

Biochem
Journal

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Vol. 7, Issue 2, Part A (2023)

In vitro cell selection and multiplication of Fusarium oxysporum f. sp. dianthi insensitive calli in carnation cultivars Tempo and Raggio-de-Sole

Author(s): Sheetal Dogra
Abstract:
In present investigation, for the development of Fusarium tolerant varieties in carnation, highly susceptible cultivars viz. Tempo and Raggio de Sole were used. Calli of caraation cultivars were irradiated to different doses of gamma irradiation. And Callus surviving at highest dose of gamma rays was mass multiplied on callus multiplication medium (MS medium +10 μNAA + 10 μM kin). Irridiated calli after multiplication, was treated with different concentrations of culture filtrate of Fusarium oxysporum f.sp. dianthi. 20% Culture resulted Fusarium wilt insensitive mutants at the end of 3rd selection cycle.
Pages: 01-04  |  423 Views  162 Downloads


International Journal of Advanced Biochemistry Research
How to cite this article:
Sheetal Dogra. In vitro cell selection and multiplication of Fusarium oxysporum f. sp. dianthi insensitive calli in carnation cultivars Tempo and Raggio-de-Sole. Int J Adv Biochem Res 2023;7(2):01-04. DOI: 10.33545/26174693.2023.v7.i2Sa.178
International Journal of Advanced Biochemistry Research
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