The enzyme known as invertase catalysis the conversion of sucrose into glucose and fructose (EC 3.2.1.26). The best source of invertase is yeast. Internal and exterior in vertases are the two varieties found in S. cerevisiae. Invertase catalyses the hydrolysis of sucrose into fructose and glucose. Pharmaceuticals, analytical measuring probes, and yeast species detection ELISA kits all include this invertase. Immobilization of the invertase naturally is what is meant by this. Different levels of glycoprotein polymerization, glycosylation, and phosphorylation make bacteria invertase unique. A range of gram-positive and gram-negative organisms, including Zymomonas mobilis, and Bacillus strains, produce invertase. Comparing fungal growth to the time course of invertase synthesis by fungi, which ranged from 24 to 192 hours, was analysed. Twenty-four-hour intervals were used to measure the invertase activity. The biological characteristics that were looked at in this study included the amount of invertase-producing bacteria and soil invertase activity. Invertase is used to replace traditional acid hydrolysis.