Breast cancer is the commonest malignancy and principal cause of women’s death. It is more common after menopause. The women are more likely to have biologically aggressive breast cancer, such as higher-grade tumors and more lymph node involvement, thus resulting in a poor prognosis, which results in increased mortality rates. In the study, thirty diagnosed breast cancer patients and thirty controls are selected. The information regarding all patients is taken in a data sheet. Breast cancer women have received chemotherapy treatment. The study reveals no significant difference (p>0.05) in RBS (Random Blood Sugar), serum cholesterol and serum estrogen levels in the case and control group. ESR (Erythrocyte Sedimentation Rate), serum calcium, HDL (High Density Lipoprotein), LDL (Low Density Lipoprotein) and serum progesterone levels show a significant difference (p<0.05) between female breast cancer patient and control group. Chemotherapy treatments may result in increasing or decreasing level of biochemical profile. The study of serum biochemical profile should be helpful tools in the monitoring of disease, metastasis, different treatment strategy and survival rates of breast cancer patients.