The treatment of infected wound healing with plant secondary metabolite from herbal plants is an active researcher in biomedicine. The Phyto-molecules or secondary metabolites in the plant extract can target cellular and molecular levels in various stages of the wound repair process. These molecules have multiple pharmacological activities that effectively accelerate wound repair and regeneration. The most common mechanism of action of natural products on the wound repair process is established through in vitro and in vivo studies. This includes direct action on skin cells regeneration, increase in connective tissue deposition such as collagen, anti-oxidant properties, modulation of cytokine and growth factor production and/or function, as well as anti-inflammatory action. The wound healing process is characterized by dynamic, interacting activities involving soluble mediators, blood cells, extracellular matrix, and parenchymal cells that culminate in permanent restoration of anatomical function and functional integrity. Chronic wounds such as infected wounds and diabetic foot ulcers suggest a failure in some aspects of the repair process. To assess the healing potential of a variety of natural products, in vivo and in-vitro assay models are utilized. Based on these assessments, many new therapies that target various stages of wound repair are emerging in recent years. Amongst them, extracts of plants from traditional medicine are beneficial for treating infected wounds. The present review paper on Triphala ointment aimed to outline the wound healing potential of Triphala and its other pharmacological activities and review its bioactive compounds, which have beneficial effects on wound regeneration activity. Furthermore, Animal studies are extensively used for our critical review and these potential data are used to further substantiate the therapeutic potential of Triphala ointment via histological and biochemical studies.