The objectives of this study are to: (i) qualitatively and quantitatively assessing the chemical components of Lawsonia inermis
Leaves from cultivated plants in two localities in Central Sudan, and (ii) to identify of their chemotypes by using GC/MS.
Materials and Methods: The cultivated plant Lawsonia inermis L. in Ed-Damer and AL-Fetaehab regions were collected in October 2014 from Ed-Damer city, Nile River State (Ed-Damer sample) and Omdurman South, Khartoum State (AL-Fetaehab sample). All plant samples under study were separately extracted by ethanol (80%) at room temperature for 24 hours. After extraction it were filtered and then the removal of solvent was done. The qualitative and quantitative analysis of the samples were carried out by using GC/MS technique. Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST).
Results: The ethanol extract of leaves of L. inermis (AL-Fetaehab sample) showed that the D-allose (17.61%), lawsone (12.87%), beta-D-glucopyranoside, methyl (12.74%), phytol (10.78%), 1-isobutoxy-1-methoxypropane (9.18%), n-hexadecanoic acidin6.33%), 9,12,15-octadecatrienoic acid (Z,Z,Z) (4.44%), squalene (4.06%) and vitamin E (3.60%) as the major phytochemical constituents, whereas the ethanol extract of leaves of L. inermis (Ed-Damer sample) showed that the compounds: beta-D-glucopyranoside, methyl (36.10%), phytol (10.85%), 9,12,15-Octadecatrienoic acid, Z,Z,Z (7.31%), squalene (7.20%), vitamin E (6.82%) and lawsone (6.79%) as the major phytochemical constituents.
Conclusion: According to the chemical composition of Lawsonia inermis, it can be classified as (i) lawsone (naphtha Quinone) Chemotype (AL-Fetaehab sample) and (ii) ethyl alpha-d-glucopyranoside (Phenolic glucoside) Chemotype (Ed-Damer sample).