International Journal of Advanced Biochemistry Research
Vol. 2, Issue 1, Part A (2018)
Synthesis of cinnamyl alcohol acetate in a static lipase bioreactor at room temperature
Author(s): Xiaohong Wang, Fangdi Cong, Yaling Fan, Jinnan Lin and Shulin Zhang
Abstract: To make the synthesis of cinnamyl acetate not only green but also low energy consumption, absorbent cotton was taken as an immobilized material to prepare the immobilized Pseudomonas fluorescens lipase in a column glass bottle and obtain a simple immobilized lipase bioreactor, and employed in catalysis of vinyl acetate reacting to cinnamyl alcohol. It is interesting that, even at room temperature and under static state, the bioreactor still behaves a good catalytic activity, and the proper addition of acylation reagents or solvents in the bioreactor can increase the reaction conversion. The best reaction system is composed of 3 mL vinyl acetate and 1.34 g cinnamyl alcohol. After 24 h, the molar conversion can reach 80.0%, and after 48 h, it can reach 91%. Obviously, the immobilized lipase bioreactor has an obviously catalytic effect of low energy consumption, and has potential value for further development and application in industry.